The 53-year-old Material Girl also displayed a photo of a man resembling Nazi leader Adolph Hitler during the song "Nobody Knows Me."
“This is just another provocation in Madonna’s world tour so that people will talk about her,” National Front vice-president Florian Philippot told BBC. “Marine Le Pen will defend not only her own honour but her supporters and the millions of National Front voters.”
Madonna played Saturday in Paris, but has used the image elsewhere during her MDNA world tour.
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The National Front will present its lawsuit this week, Philippot told Reuters.
Madonna’s world tour is also using images of Pope Benedict XVI and toppled Egyptian leader Hosni Mubarak, although without the Nazi imagery.
The National Front threatened legal action after the image appeared earlier in Madonna’s tour, RFI reported.
The National Front won 18 percent of the vote during this year’s presidential elections, sending two representatives to 577-seat national assembly.
Le Pen is attempting to broaden the National Front’s appeal by distancing itself from hardliners.
Madonna will play Nice, France, on Aug. 21.